Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC MOPS | 1132-61-2 | 99.9% | 100 G
MOPS is a buffering agent used in biology. MOPS buffer maintains the pH of mammalian cell culture media. It also significantly improves the thermal stability of BSA (HY-D0842).
Molecular Dimensions 100 ML BOTTLE OF 1M MES
100 mL bottle of 1M MES (2-(N-Morpholino)ethanesulfonic acid monohydrate)
Medchemexpress LLC HEPES | 7365-45-9 | 99.9% | 1 G
HEPES is a nonvolatile zwitterionic chemical buffering agent broadly applied in cell culture. It is effective at pH 6.8 to 8.2 and is also a potent inducer of lysosome biogenesis.
- Nonvolatile zwitterionic chemical buffering agent
- Broadly applied in cell culture
- Effective at pH 6.8 to 8.2
- Potent inducer of lysosome biogenesis
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 50 G
2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 50 G
Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 25 G
MOPS sodium salt is a buffering agent utilized in biological applications. MOPS buffer is effective in maintaining the pH of mammalian cell culture media and has been shown to significantly improve the thermal stability of BSA. It is relatively safe for short-term experiments, though it may reduce cell viability after 4 hours, suggesting experimental duration limits of 4-12 hours.
- Buffering agent for biological research.
- Maintains pH in mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
- Relatively safe for short-term experiments, though it may reduce cell viability after 4 hours, suggesting experimental duration limits of 4-12 hours.
- Can be used in the preparation of keratinocyte and microbial culture media.
Alkali Scientific CellPro™ DPBS Buffer, 1X, with Calcium and Magnesium, 1L, 10/Case
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Medchemexpress LLC MES hydrate | 1266615-59-1 | MFCD00149409 | 99.9% | 10 G
MES (2-Morpholinoethanesulphonic acid) hydrate is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is classified as a Good's buffer and is widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies. This zwitterionic buffer provides effective buffering capacity around pH 6.15. Its pKa value changes with temperature, a factor to consider when used at varying temperatures. MES hydrate exhibits good buffering properties in biological experiments and has a poor affinity for metal ions, making it suitable for studies involving such ions.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity at approximately pH 6.15
- Poor affinity with metal ions
Medchemexpress LLC 4-Morpholineethanesulfonic acid, potassium salt | 39946-25-3 | 99.9% | 10 G
MES potassium (2-Morpholinoethanesulphonic acid potassium) is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. As a Good's buffer, it is utilized in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies. This zwitterionic buffer provides effective buffering capacity around pH 6.15 and shows poor affinity with metal ions, making it suitable for studies involving them.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity at around pH 6.15
- Poor affinity with metal ions, making it suitable for studies involving metal ions
Medchemexpress LLC 1,4-Piperazinedipropanesulfonic acid, β1,4-dihydroxy- | 68189-43-5 | ≥95.0% | 25 G
POPSO is a zwitterionic buffer that increases osmolality and demonstrates significant inhibition of anion uniport. It inhibits chloride uniport with an IC50 value of 24 mM. POPSO also enhances copper uptake and toxicity in alga and impairs the mitochondrial inner membrane. The working pH range for POPSO sesquisodium salt is 7.2-8.5.
- Zwitterionic buffer
- Increases osmolality
- Marked inhibition of anion uniport
- Inhibits chloride uniport with an IC50 value of 24 mM
- Enhances copper uptake and toxicity in alga
- Impairs mitochondrial inner membrane
- Working pH range (for sesquisodium salt): 7.2-8.5
Perkin Elmer US LLC ELAN 9000/6X00/DRC-e Wash Solution, 1% HNO3, 1,000 mL
Concentration of Element includes: Matrix Volume 1 % HNO3 1,000 mL