Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
HIMEDIA Dulbeccos Phosphate Buffered
Dulbecco s Phosphate Buffered Saline 1X w/o Phenol red Calcium and Magnesium
TRIBIOSCIENCE INC Inoue Transformation Buffer, 500ML
Inoue Transformation Buffer is used for the preparation of high-efficient competent cells. This product is based on the Inoue method for the preparation and transformation of E. Coli component cells.
Teknova 1X PBS Solution, pH 7.4. 100mL, Sterile. 10 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Rockland Immunochemicals 10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride) (4 x 1 liter) - MB-008-4000
Phosphate buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.
MSC Value Collection Magnifier, 20x, Dual Lens, Round, Metal
The Value Collection magnifier is a durable tool designed for detailed inspection. It features a high 20x magnification, a dual-lens system, and a robust metal construction, making it suitable for various precision tasks.
- 20x maximum magnification
- Dual lens system
- Round lens shape
- Metal construction
PLANT CELL TECHNOLOGY 1X Dulbecco's Phosphate Buffered Saline Solution without Calcium and Magnesium, Buffer Solution, Mod. 1, Clear Liquid, Sterile, 500mL bottle.
1X DPBS Mod. 1 is a sterile phosphate-buffered saline solution for mammalian cell culture applications. Provides isotonic environment to maintain cell integrity during washing, resuspension, or media preparation. Supplied as a clear liquid in a 500 mL sterile bottle. Ready-to-use and shelf stable under recommended conditions. Suitable for research, cell culture, and tissue engineering workflows.
Teknova 1X PBS with 0.1% Tween-20. 1000mL, Sterile. 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Apexbio Technology LLC HyperScript RT SuperMix for qPCR (with gDNA wiper) 50 rxns
HyperScript RT SuperMix for qPCR is a reverse transcription reaction premix based on HyperScript Reverse Transcriptase a novel enzyme engineered from M-MLV (RNase H-) Reverse Transcriptase featuring significantly reduced RNase H activity and greatly enhanced thermo-stability Due to its improved heat tolerance HyperScript Reverse Transcriptase is especially suitable for reverse transcription of RNA templates with complex secondary structures HyperScript RT SuperMix for qPCR (with gDNA wiper) is specifically designed for two-step qRT-PCR assays The 5 SuperMix contains all necessary components for reverse transcription Simply adding template RNA and RNase-Free ddH2O allows rapid reaction initiation The included 4 gDNA wiper efficiently eliminates potential genomic DNA contamination from samples rapidly and thoroughly prior to reverse transcription The kit is particularly suitable for reverse transcription reactions from RNA templates of low concentration
Alkali Scientific QuickSilver™ PBS Buffer Powder, 100 Pouches
QuickSilver™ Buffer Powder packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, and easy to use. Convenient premeasured pouches make 1L of 1X buffer. There is no pH adjusent needed and they offer a long shelf life. They come supplied with preprinterd bottle labels for easy identification. 100 pouches per pack.