Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1X PBS Solution, pH 7.5. 2L, Sterile 2 pack.
137.0mM Sodium Chloride, 2.7mM Potassium Chloride, 4.3mM Disodium Phosphate, 1.4mM Monopotassium Phosphate
Teknova Tris Glycerol Buffer pH 8.0. 1000mL, Endotoxin Tested, Sterile 2 pack.
20mM Tris,25mM Sodium Chloride,2.5% Glycerol
Teknova 2M Tris-HCl, pH 7.5 Solution. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova RIPA Buffer Solution. 500 mL, Sterile. 4 pack.
150mM Sodium Chloride, 1.0% Triton X-100, 1.0% Sodium Deoxycholate, 0.1% Sodium Dodecyl Sulfate, 50mM Tris-HCl,2mM Ethylenediaminetetraacetic acid (EDTA)
Teknova Organic Sperm Wash Buffer. 1000mL, DNase Tested, Sterile. 8 Pack.
50mM Sodium Chloride, 10mM Tris-HCl, 10mM Ethylenediaminetetraacetic acid (EDTA), 2.0% SDS
Teknova 10X PBS Solution. 1000mL, Sterile. 2 pack.
1.35M Sodium Chloride,27mM Potassium Chloride,43mM Disodium Phosphate,14mM Monopotassium Phosphate
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.6) - 4L
Phosphate Buffered Saline PBS (10X pH 7 6) - 4L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Sodium Cacodylate Buffer (0.1 M, pH 6.5) - 100ml
Sodium Cacodylate Buffer (0 1 M pH 6 5) - 100ml (For Research Use and Further Manufacturing Only)
![Research Products International Corp ACES [N-(2-Acetamido)-2-aminoethane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-A22500-100.0.JPG-250.jpg)
Research Products International Corp ACES [N-(2-Acetamido)-2-aminoethane], 100 Grams
Zwitterionic buffer used in biochemistry and molecular biology research.
- pKa (25°C): 6.88
- The working pH range in aqueous solution is 6.1 - 7. 5
- Absorbance @ 280nm (5% water): <0.02
![Research Products International Corp Ethylenediaminetetraacetic acid [EDTA], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-E58100-500.0.JPG-250.jpg)
Research Products International Corp Ethylenediaminetetraacetic acid [EDTA], 500 Grams
Substance Name: EDTA
CAS Number: 60-00-4
Molecular Formula: C10H16N2O8
Molecular Weight: 292.24
Chemical Purity: >99%
Soluble in: Water
Store at: Room Temperature
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4) - 1L
Tris-HCl Buffer (1 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)